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KMID : 0357819770010010101
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Korean Journal of Legal Medicine
1977 Volume.1 No. 1 p.101 ~ p.106
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Agglutination of Extract of Clerodendron trichotomum Thunberg with Animal Red Blood Cells
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李亨柱/Rhee, Hyung Ju
李鳳熙/黃迪駿/Lee, Bong Hee/Hwang, Juck Joon
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Abstract
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Moon and Wieners´ had demonstrated new blood factor "Cl" with anti-Cl lectin prepared from extracts of seeds of korean Clerodendron trichotomurn Thunberg by absorption of selected human red blood cells.
The purpose of this papers is to study on the distribution of CI type in animal blood and to know the possibility to prepare anti-Cl lectin by absorption of animal red blood cell too.
Therefore, agglutinations of extract of korean Clerodendron trichotomum Thunberg and anti-Cl lectin to various animal red blood cells such as rabbit, mouse, rat, guinea pig, chicken, dog, hog, bovine, goat, sheep, horse and cat were carried out. Then, absorption by animal red blood cells and agglutination inhibition by various sugars were performed, and the results can be summarized as follows;
Animal red blood cells such as rabbit, rat, chicken, hog, horse and cat were agglutinated by Clerodendron extract. And anti-Cl lectin agglutinated the red blood cells of rat, chicken, hog, horse and cat. By absorption of Clerodendron extract with the selected red blood cells of chicken, anti-Cl lectin can be also ;prepared as with human red cells.
Agglutination activities of extract of Clerodendron to horse red blood cells was inhibited by all of the used sugars already mentioned in the materials, but to rabbit and chicken was not by all of the used sugars. And the agglutination activities" of Clerodendron extract to red blood cells of rat, hog and- cat were not by lactose and D-galactose.
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